Emmental cheese. Credit score: Wikipedia

Researchers on the Technical College of Munich (TUM), the Leibniz-Institute for Meals Programs Biology, and the College of Hohenheim have developed a brand new methodical strategy for the sooner identification of flavor-giving protein fragments in meals reminiscent of cheese or yogurt, thus optimizing manufacturing processes.

The style of fermented meals reminiscent of cheese, yogurt, beer, yeast dough, or soy sauce could be very fashionable with customers worldwide. Along with risky aroma compounds, non-volatile substances additionally considerably contribute to their attribute style profile. Above all, these embrace fragments of lengthy protein molecules which are produced, for instance, throughout microbial or enzymatic conversion (fermentation) of milk or grain protein.

At current, nonetheless, it’s nonetheless unclear which of the greater than 1000 protein fragments in fermented milk merchandise are liable for the flavour, one purpose being that beforehand used analytical strategies are very labor-intensive and time-consuming.

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New analytical strategy supplies an answer

A staff of scientists led by Thomas Hofmann, head of the Chair of Meals Chemistry and Molecular Sensory Science at TUM, has developed a brand new analytical strategy to handle this downside. What makes the strategy so progressive is that researchers mixed present strategies of proteome analysis with strategies of sensory analysis to effectively and shortly establish the decisive flavor-giving protein fragments from the totality of all fragments. “We coined the time period ‘sensoproteomics’ for the sort of process,” mentioned Andreas Dunkel from the Leibniz-Institute for Meals Programs Biology, the lead researcher for the research.

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The scientists examined the effectiveness of the newly developed process for the primary time on two completely different sorts of cream cheese with completely different levels of bitterness. The target was to establish the particular protein fragments liable for a bitter off-flavor in cheese that happens underneath sure manufacturing circumstances.

An strategy to cut back 1,600 prospects to 17

The researchers began their work with an in depth evaluation of the literature. They concluded {that a} complete of roughly 1,600 protein fragments contained in dairy merchandise may theoretically be liable for the bitterness. Subsequent liquid chromatography-coupled mass spectrometer investigations assisted by in-silico strategies decreased the variety of potential protein fragments to 340. Lastly, comparative spectrometric, sensory and quantitative analyses decreased the variety of fragments liable for the bitter cheese taste to 17.

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Prof. Hofmann, who can be the Director of the Leibniz-Institute for Meals Programs Biology at TUM, says, “The sensoproteomics strategy we’ve developed will, sooner or later, contribute to the fast and environment friendly identification of flavor-giving protein fragments in a variety of meals utilizing high-throughput strategies—a big assist in optimizing the style of merchandise.”

Discover additional:
Gooda, Gouda! Fixing the 800-year-old secret of an enormous cheese

Extra info:
Karin Sebald et al, Sensoproteomics: A New Strategy for the Identification of Style-Lively Peptides in Fermented Meals, Journal of Agricultural and Meals Chemistry (2018). DOI: 10.1021/acs.jafc.8b04479

Journal reference:
Journal of Agricultural and Meals Chemistry

Supplied by:
Technical College Munich


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